Subproject 2.1


Advances for recombinant FSH development; Studies based on transgenesis and somatic cell nuclear transfer technologies of farm animals


The general objective of the current sub-project is to improve the transgenic cloning of farm animals used for manufacturing of therapeutically valuable human proteins. We exploit rhFSH as a model to advance the complete pipeline of techniques such as construction of rhFSH vector, transfection of bovine mammary epithelial cells, HTP bioactivity characterisation of hormone isoforms and the use of transgenic cells in SCNT to obtain FSH-transgene bovine embryos.

Project heads: Prof. Sulev Kõks, prof. Ülle Jaakma