New ultrasensitive biosensors for embryo selection
The objective of the sub-project is to develop new ultrasensitive biosensors for human and animal non-invasive embryo selection by analysing embryo’s secretome from culture medium. Biosensors will be designed for previously known embryo quality biomarkers (human HCG and HLA-G) as well as newly identified peptidomic and proteomic markers from subproject 2.2. using ultrasensitive modified total internal reflection fluorescence, TIRF-based biosensors for simultaneous and on-line monitoring of multiple markers. The benefits of the novel tests will be evaluated in retrospective and prospective studies using human and bovine elective single embryo transfers.
Project head: Prof. Ago Rinken